Development and Characterization of EST-SSR Markers in Bombax ceiba (Malvaceae)
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چکیده
منابع مشابه
Development and characterization of EST-SSR markers in Bombax ceiba (Malvaceae)1
PREMISE OF THE STUDY Bombax ceiba (Malvaceae), commonly known as silk cotton tree, is a multipurpose tree species of tropical forests. Novel expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized for the species using transcriptome analysis. METHODS AND RESULTS A total of 33 new EST-SSR markers were developed for B. ceiba, of which 13 showed polymorph...
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Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....
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In this study, a cDNA library was constructed from the total RNA of sweet potato leaves. A total of 789 copies of the cDNA were cloned in Escherichia coli by employing the pGEM-T Easy vector. Sequencing was carried out by Solgent Co. (Korea). As many as 579 expressed sequence tag-simple sequence repeat (EST-SSR) markers were designed (73.38%) from the known cDNA nucleotide base sequences. The l...
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ژورنال
عنوان ژورنال: Applications in Plant Sciences
سال: 2015
ISSN: 2168-0450
DOI: 10.3732/apps.1500001